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KMID : 0861020100250040077
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Korea Journal of Herbology
2010 Volume.25 No. 4 p.77 ~ p.84
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Anti-inflammatory effect and contents from the aerial part and root of the various Taraxacum spp. distributed in Korea
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Lee Mi-Hwa
Song Sun-Ho
Ham In-Hye
Bu Young-Min
Kim Ho-Cheol
Choi Ho-Young
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Abstract
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Objectives: Taraxaci Herba et Radix (THR) is widely used as a food and medicinal herb in Korea. It has been used for treatment of virus inflammatory disease, liver diseases and gastritis. So far, anti-inflammatory effects and constituents of various species in THR has not been studied for comparison. The aim of this study is to compare the anti-inflammatory effects of the aerial part and root from various THR. Also, we have compared the contents of its known constituents with each.
Methods: In this study, we estimated anti-inflammatory effect and compared their constituent by HPLC. For the determination of anti-inflammatory effects, we investigated NO and PGE2 production by ELISA. The expressions of iNOS was determined by western blotting in LPS?induced RAW 264.7 macrophage cells. And, standard compounds which are methyl gallate, gallic acid, syringic acid and esculetin of THR were analyzed by HPLC using a C18 column.
Results: Methanol extracts of THR decreased NO and PGE2 production. The expressions of iNOS protein were also decreased in methanol extracts of THR. As a result, HPLC analysis showed that they showed similar patterns. Methyl gallate and esculetin showed the highest content. Methyl gallate was included over 10% content in each aerial part and root of THR.
Conclusions: These results indicate that most of THR distributed in Korea might represent therapeutic agent for treatment of inflammatory diseases.
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KEYWORD
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Taraxacum, inflammation, nitric oxide, prostaglandin E2, HPLC
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